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酵素
酸化還元酵素　EC.1
rD-LDH (L)

rD-LDH (L)

rD-LDH (L)
D-Lactate Dehydrogenase (Recombinant from Leuconostoc sp.)
組換えD-乳酸脱水素酵素（Leuconostoc sp由来）

反応	D-Lactate + NAD+　⇄　Pyruvate + NADH + H+
規格	Specific activity IU/mg protein > 1000 units Contaminants Malate dehydrogenase < 0.01% Myokinase < 0.02% Pyruvate kinase < 0.003% Glutamic-pyruvic transaminase* < 0.001% Glutamic-oxaloacetic transaminase* < 0.001% *α-Hydroxyglutarate dehydrogenase activation included.
分析方法	I. Spectrophotometric Method Wavelength: 340 nm, Light path length: 1 cm Final volume: 3.17 mL, Temperature: 25ﾟC Pipette the following reagents into a cuvette 3.00 mL K-phosphate buffer (0.1 mol/L, pH 7.0) 0.10 mL Na-pyruvate (25.4 mmol/L) 0.05 mL NADH (10 mg/mL) dissoluted in Tris (10 mmol/L) 0.02 mL rD-LDH(L) (approx. 3 IU/mL) II. Calculation IU/mL = ΔA/min・V・D 6.3・d・v ΔA/min : The change in absorbance at 340 nm/minute(reverse the blank activation of rLDH(PH)(-)) V : Total volume of reaction mixture (3.17 mL) D : Enzyme dilution factor 6.3 : mmol/L extinction coefficient of NADH (L･mmol-1･cm-1) d : Light path length (1 cm) v : Volume of enzyme sample (0.02 mL)
基礎データ
形態/保存	Product Code：rD-LDH(L)-83 Lyophilized powder 冷凍（-20℃以下）

関連キーワード

体外診断薬、生化学、酵素、ALT、AST、GOT、GPT

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rD-LDH(L)

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